Hi @Shyam Narayan ,
Is there any error message screenshot when you sign in Skype for Business?
If client could connect to skype for business server, DNS is OK, secure TLS connection could establish successfully, you should check the following things:
1.Client should trust the Certificate issued on the Server (Client should contain the Root/Intermediate Certs)
2.FQDN/ URL that we are trying to access should be part of Certificate SAN entry on the Server where we are connecting to.
3.Required TLS Protocol & TLS cipher suites should be allowed in Firewall (If any)
For more details about how to troubleshoot sign-in issue, you can refer to:
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