Hi,
Sorry I need to ask a few questions to get some more information.
1.What version of Exchange are you running?
2.Is it a fresh install? If not,what changes have been done recently before the problem appears?
Here are some suggestions:
1.Try recycling the MSExchangeECPAppPool in the IIS manager-->Application Pools
2.Recreate the EAC virtual directory via running the following commands in EMS(Exchange Management Shell)
Remove-EcpVirtualDirectory -Identity "Server01\ecp (Default Web site)"
New-EcpVirtualDirectory