Hi ,
First check the authentication method on server and client. If you’ve made any changes to the default settings for IKEv2 cryptography settings, those must match on the client and VPN server.
Before we go further, I would like to confirm the following questions:
1.If you change to SSTP connection, will it still failed to connect?
2.What's the OS version of your windows server? server 2016 or server 2019?
In fact, the error message is very general and it is hard for troubleshooting without tracing network traffic. We need to trace network monitor to see what is actually occurring on the network when Always On VPN stopped working.
However, analysis of network traffic is beyond our forum support level and due to forum security policy, we have no such channel to collect user log information. So we recommend you open a case with MS Professional tech support service, they will help you open a phone or email case to Microsoft, so that you would get a technical support on a one-to-one basis while ensuring private information.
Here is the link:
https://support.microsoft.com/en-us/gp/customer-service-phone-numbers
In addition, check if the following article is helpful with you:
Always On VPN and IKEv2 Fragmentation
Best Regards,
Candy
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